Fig. 1
Immunohistochemical survey of L-plastin expression in early zebrafish development.
A. Enveloping layer (EVL) expression at 50% epiboly, seen in equatorial view.
B. EVL expression at 50% epiboly, seen in cutaway view of a single focal plane. The squamous EVL is strongly stained, in contrast to the deep cells (DC) and the yolk syncytial layer (YSL).
C. EVL expression at 90% epiboly, seen obliquely from the vegetal pole.
D. Periderm expression at the somite stage. Stain intensity is variegated, appearing brighter or darker in adjacent cells.
E. 70% epiboly, intermediate magnification of animal cap EVL.
F. 70% epiboly, high magnification of animal cap EVL. In this single focal plane, L-plastin expression appears as tightly packed cytoplasmic dots or clumps.
DC = deep cells; EVL = enveloping layer; YSL = yolk syncytial layer.
Reprinted from Gene expression patterns : GEP, 32, Baumgartner, E.A., Compton, Z.J., Evans, S., Topczewski, J., LeClair, E.E., Identification of regulatory elements recapitulating early expression of L-plastin in the zebrafish enveloping layer and embryonic periderm, 53-66, Copyright (2019) with permission from Elsevier. Full text @ Gene Expr. Patterns