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Fig. 10
Targeted photo-stimulation of Mauthner cells evokes tail curling in larval zebrafish. (a) Tail movement of head-restrained larval zebrafish measured in terms of angle between current tail direction (blue) and resting tail position (red). (b, c) Example showing that targeted photo-stimulation (b, yellow triangle) of ChrimsonR-expressing Mauthner cell (b, red) reliably evoked tail curling in five consecutive trials (c). White dashed lines in (b): eye outlines; yellow shaded areas in (c), photo-stimulation periods. (d, e) Example showing that targeted photo-stimulation (d, yellow triangle) of contralateral Mauthner cell (d, red dash circle) with expression of GFP but not ChrimsonR could not evoke tail movement (e). The data shown in (b)−(e) were obtained from the same larva. (f) Success rates of tail movements evoked by targeted photo-stimulation of unilateral Mauthner cells with (red) and without (green) ChrimsonR expression. The numbers indicate the number of examined cells.