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Fig. 3
Wound recruited macrophages of 0, 3, 9, 18 J/cm2 treatment groups of fish (n = 20, 12, 10, and 8, respectively) were tracked using software analysis. Representative control fish (A) and 3 J/cm2(B) are shown with trajectories with the highest total distances hued as dark red and lowest values hued as dark blue. Quantitative measures were algorithmically derived at T1 (30–60 min postinjury), T2 (30–90 min postinjury), and T2 (30–120 min postinjury) temporal domains postinjury with means and 95% CIs for each independent group measure. Macrophage measures of absolute velocity (C)meandering index (D) were determined to evaluate persistence. Wound-oriented net distance traveled (E) and static ratio (F)were determined to evaluate wound bias efficiency. Statistical differences were computed with Student's t-tests (*p < 0.05), (**p < 0.01) in Graphpad® Prism.