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Fig. 7
HAB reveals the dynamics of neutrophil granules upon phagocytosis of zymosan particles in live zebrafish. (a and b) Confocal live imaging of HAB-labeled neutrophil granules upon phagocytosis of subcutaneously injected zymosan in a live 72 hpf zebrafish larva under diffusion conditions. HAB is recruited to the forming phagosomes (arrows). Inset: HAB labeling of a resting neutrophil. (c) Sudan Black (SB) staining of myeloperoxidase-containing neutrophil granules showing granule recruitment to the phagosome upon zymosan phagocytosis in fixed zebrafish larvae. Inset: SB staining of a resting neutrophil; a single 1 μm optical section is shown. (d) Frames extracted from an in vivo time-lapse confocal imaging sequence (time step = 1 min). Arrows point to HAB-labeled neutrophil granules that are recruited to the nascent zymosan containing phagosome. Three neutrophils (pointed with number 1 to 3) were tracked during the time lapse sequence. Maximum intensity Z-projection (1 μm serial optical sections). See ESI for Video S8† related to (d).