Figure 4 - figure supplement 1

Figure 4 - figure supplement 1 Developmental NTN1 expression profiling in chick eye and OF.

(a) Whole-mount in situ hybridisation revealed NTN1 expression in the ventral eye, developing pharyngeal arches, and otic vesicles at HH.St22. Enlarged panels showed regionally-restricted NTN1 expression in the developing fissure margins (arrows) at HH.St22 (Top) and HH.St24 (Bottom). (b) Section colourimetric in situ hybridisation and NTN1 immunofluorescence analyses at HH.St28 showed NTN1 expression was specific to the edges of the early medial OFM immediately prior to fusion. (c) (Top panels) RNAscope analyses at FP1/distal iris-region OFM at HH.St29 showed NTN1 mRNA specificity (arrows) in the fissure margin and a graded reduction in the fused seam. (Bottom) Positive control analyses for RNAscope showed strong NTN1 mRNA signals in the basal floorplate of the neural tube at HH.St29. The OFM midline is shown by a yellow arrowhead in all panels.