Figure 3—figure supplement 1.

Figure 3—figure supplement 1.

The biological validation of imaging resolution was performed through measurement of distances between Z-lines in the swim bladder striated muscle strands. Cross sections of striated muscle surrounding the swim bladder diagonally in larval (five dpf) zebrafish (A) was used to generate pixel intensity profiles (B). Measurement of pixel distances between local intensity maxima assuming 0.743 μm³ isotropic voxel resolution yielded an average sarcomere length of 2.16 μm (SD = 0.55 μm for 293 distance measurements), consistent with published sarcomere lengths in larval zebrafish (Burghardt et al., 2016; Dou et al., 2008).