Fig 6

Fig 6

(A) Transcripts of members of the Hippo signalling pathway fat2, lats2 and yap1 were less abundant in tfap2a;tfap2c mutants when compared to wild-type siblings. A schematic showing their role in signal transduction and transcription inside a cell. (B) CRISPR/Cas9 mutations were made in the first exon of yap1 leading to the two alleles described. The exon-intron structure of the yap1 transcript is shown in gold. The exact deletions are displayed below. (C) Embryos from a single clutch were split and raised at 24C and 31.5C. Bars indicate the absolute number of fish forming a swim bladder at 5 dpf for each yap1^sa25458 genotype (D) Normalised counts of 3’ tag sequencing data at 24 hpf comparing yap1^sa25458 mutants to wild-type siblings. All four genes, yap1, gch2, wu:fc46h12 and padi2, have an adj. p-value <0.05. (E) Maternal zygotic yap1 mutants present a strong reduction in melanocyte numbers at 36 hpf at both dorsal (arrow head) and ventral tail regions (arrow). (F) Quantification of melanocytes with the quantities on the left and then broken down into the regions of the head, yolk, ventral tail and dorsal tail. Each dot represents a region in a single larva, siblings in blue and MZyap1^sa25458 in red. A statistical significance of <0.05 is indicated with “*”.