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FIGURE 6

ID: ZDB-IMAGE-190723-846
Publication: Nelson et al., 2019 - Glucocorticoids Target Ependymal Glia and Inhibit Repair of the Injured Spinal Cord
All Figures: Figures for Nelson et al., 2019

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FIGURE 6

Glucocorticoids suppress neurogenesis. (A–C) Whole-mount preparations (lateral view) of Tg(gfap:EGFP) transgenic zebrafish show immunostaining for EGFP (green) and HuC/D (neurons, blue) along with detection of EdU (red) from 120 h post EdU incubation and sham injury (A), SCI followed by no treatment (B), and +Dex (C). Dashed boxes represent a 200-μm region centered at the lesion site used for quantifying cell counts and serve as scale bars (A is the same as B,C). (D) Quantification of the mean number of EdU-positive cells within the spinal cord (± SEM). ^∗p < 0.05; ^∗∗p < 0.01; ns, not significant; 10 whole mounts per condition.

Acknowledgments

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