Fig. 6

Fig. 6

Voltage-clamp recordings of fast and slow muscles in control and En1-DTA fish. a A schematic illustration of the simultaneous voltage-clamp recordings of two muscle cells (one for a fast muscle, the other for a slow muscle). Neuro-muscular transmission was partially blocked in these experiments, and portions of synaptic currents remained in the recording conditions. b An image after a recording. Slow and fast muscles were morphologically distinct and could be easily distinguished. Scale bar, 20 μm. c Example of simultaneous voltage-clamp recordings in control fish. d Example of simultaneous voltage-clamp recordings in En1-DTA fish. e Normalized currents in the slow muscle cells in the cycle when the fast muscle received strong inputs (>50% of the maximum). Five paired recordings were performed. Control: 0.44 ± 0.08, En1-DTA: 0.73 ± 0.04. Data are mean ± s.d. **P < 0.01 (two-tailed t test, P = 0.00091)