Fig. 1

Fig. 1

a1–C3: Proliferative cells labeled with BrdU (red), SCs labeled with Sox2 (white), and HCs labeled with GFP (green). a1–A3: Control neuromasts; b1–B3: After addition of BIO; c1–C3: After addition of IWR-1. d: Quantification of proliferative cells (BrdU⁺), SCs (Sox2⁺), and HCs (GFP⁺) shown in a1–C3. e1–F3 and h1–I3: Proliferative cells labeled with BrdU (red), SCs labeled with Sox2 (white), and HCs labeled with myosin VI (green). e1–E3: wt Apc larvae; f1–F3: Apc^mcr mutants showing elevated Wnt/β-catenin activity; h1–H3: Heat shock-negative control larvae (HS-ctr); i1–I3: Wnt repression by heat shock induction of the hs:dkk1 transgene (hs:dkk1). g, j: Quantification of the proliferative cells (BrdU⁺), SCs (Sox2⁺), and HCs (Myosin VI⁺) shown in e1–F3 (g) and h1–I3 (j). n = 5–7 fish per group. ** Indicates p < 0.001, and error bars indicate the standard error of the mean. K1–O1 and K2–O2: WISH analysis of Sox2 (K1–O1) and atoh1a (K2–O2) expression in the neuromasts from different groups. Scale bar in I3 = 20 μm for a1–C3, e1–F3, and h1–I3. Scale bar in O2 = 30 μm for K1–O1, K2–O2