ZFIN Image: Aksoy et al., 2019, Fig. 2

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Fig. 2

Chemical enhancement of CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9-mediated homology-directed repair (HDR) efficiency. Effects of a SCR7, b NU7441, c RS-1 on the efficiency of CRISPR/Cas9-mediated tdTomato expression in zebrafish embryos. d Co-injection of optimized concentrations of NU7441 and RS-1 in zebrafish embryos. Error bars represent standard error of the mean. e–g Fluorescent microscopy of zebrafish embryos injected with e tdTomato donor template, f CRISPR/Cas9 with tdTomato donor template, or g CRISPR/Cas9 with tdTomato donor template and optimized concentration of NU7441 at 50 µM. Scale bars: 250 µm

Acknowledgments

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