ZFIN Image: Turcotte et al., 2019, Fig. 4.

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Figure Caption

Fig. 4.

In vivo SR imaging of the mouse brain with AO SIM. (A) Deconvolved widefield (dWF) and SIM images of dendrites expressing ChR2-GFP, a membrane label. (Scale bar: 5 $μ$ m; Inset width: 5 $μ$ m.) (B) OTFs of the SIM and dWF images in A. (C) dWF and SIM images of neurons expressing cytosolic GFP (Thy-1 line M mouse). (Scale bar: 5 $μ$ m; Inset width: 3 $μ$ m.) (D) OTFs of the SIM and dWF images in C. (E) Time-lapse in vivo SIM images showing structural dynamics of a dendrite at a depth of 25 $μ$ m in the brain of a Thy1-GFP line M mouse after KCl injection. Arrows point to highly dynamic structures. Images were normalized independently. (Scale bar: 4 $μ$ m.)

Acknowledgments

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