Fig. 2.

Fig. 2.

SIM yields spatial resolution superior to deconvolved widefield and TPEF microscopy both ex vivo and in vivo. (A–F) Images and corresponding OTFs of the same dendritic structure in a Thy1-GFP line M brain slice at a depth of 25 $\mathrm{\mu }$m obtained with different imaging modalities, all with AO: (A and D) deconvolved widefield, (B and E) deconvolved TPEF, and (C and F) SIM. (Scale bar: 5 $\mathrm{\mu }$m; Inset widths: 3 $\mathrm{\mu }$m.) (G and H) Line profiles through a spine neck and a dendritic shaft, respectively. All deconvolutions were performed with Wiener filtering. (I–N) In vivo images of neurites in a larval zebrafish brain at a depth of 100 $\mathrm{\mu }$m. Images of the same neurites obtained with (I and L) deconvolved widefield, (J and M) deconvolved TPEF, and (K and N) SIM with and without AO, respectively. Images were normalized independently. (Scale bar: 5 $\mathrm{\mu }$m; Inset widths: 3 $\mathrm{\mu }$m).