Figure 7—figure supplement 1.

Figure 7—figure supplement 1.

Larvae were transferred at 72 h AEL to 35 mm dishes containing unmodified cornmeal-molasses agar (mock) or cornmeal-molasses agar supplemented with 20 µM PBP10 and assayed for behavior responses (Figure 7) and sheath formation at 120 h AEL. The images show cora immunoreactivity from representative mock treated and PBP10-fed larvae. Magenta arrows mark examples of epidermal sheaths and blue arrows mark septate junctions; both structures are labeled by cora but can be clearly distinguished from one another. The plot depicts mean and standard deviation values for total sheath length per hemisegment in larvae of the indicated genotypes. PBP10 feeding significantly reduces the extent of sheath formation. ***p<0.001, ***p<0.01 relative to mock; one way ANOVA with post-hoc Dunnett’s test. Scale bar, 50 mm.