IMAGE

Fig. 6.

ID

ZDB-IMAGE-190723-2569

Genes

Dendra2
EGFP

Antibodies

Publication

Skvarca et al., 2019 - Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish

All Figures

Figures for Skvarca et al., 2019

Image

Figure Caption

Fig. 6.

UPHD25 treatment reduces total M1 macrophage population, but not M2 macrophage population.Tg(mpeg1:dendra2); Tg(PT:EGFP) transgenic zebrafish were used for macrophage polarization analysis. (A,B) Immunofluorescence co-stain of TNFα (red), macrophages (blue) and PT (green) in gent-AKI+DMSO (A) and gent-AKI+UPHD25 (B). PTs are outlined in white. TNFα+/mpeg+ are marked with an asterisk and TNFα−/mpeg1+ are marked with an arrow. (C) Quantification of M1 macrophage recruitment by counting TNFα+/mpeg1+ cells adjacent to the PT. Mean_{Gent-AKI+DMSO}=0.38 (N=26) vs Mean_{Gent-AKI+UPHD25}=0.15 (N=28). (D,E) Immunofluorescence co-stain of arginase-2 (red), macrophages (blue) and PT (green) in gent-AKI+DMSO (D) and gent-AKI+UPHD25 (E). Arg2+/mpeg1+ are marked with an asterisk. (F) Quantification of M2 macrophage recruitment by counting Arg-2+/mpeg1+ cells adjacent to the PT. Mean_{Gent-AKI+DMSO}=0.61 (N=26) vs Mean_{Gent-AKI+UPHD25}=0.62 (N=27). Macrophage numbers were normalized by calculating the ratio of M1 or M2 over total macrophages; i.e. (TNFα+/mpeg1+)/total mpeg1+. Data pooled from three biological replicates are shown expressed as mean±s.e.m. Two-tailed t-test: **P<0.01, ns, not significant. Scale bars: 20 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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