IMAGE

Fig. 5.

ID

ZDB-IMAGE-190723-2568

Genes

Dendra2
EGFP

Antibodies

Ab-3G8

Publication

Skvarca et al., 2019 - Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish

All Figures

Figures for Skvarca et al., 2019

Image

Figure Caption

Fig. 5.

UPHD25 treatment has no effect on neutrophil response but lowers total macrophage recruitment during early AKI phase. (A,C) Tg(cdh17:mCherry); Tg(lyz:EGFP) transgenic zebrafish were used for neutrophil analyses. (B,D) Tg(cdh17:mCherry); Tg(mpeg1;dendra2) transgenic zebrafish were used for macrophage analyses. (A,B) Immunofluorescence co-stain of PTs (red or green) and neutrophils (green) (A) and macrophages (red) (B) in gent-AKI+DMSO and gent-AKI +UPHD25. PTs are outlined in white and leukocytes are marked with arrows. (C) Quantification of neutrophil numbers showed no significance between DMSO and UPHD25 treatment groups. (D) Quantification of macrophage numbers showed a significant decrease in macrophage numbers in UPHD25 treatment group. Mean_{Gent-AKI+DMSO}=16.38 (N=69) vs Mean_{Gent-AKI+UPHD25}=12.99 (N=75). Data pooled from three biological replicates are shown expressed as mean±s.e.m. Two-tailed t-test: *P<0.05, ns, not significant. Scale bars: 20 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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