IMAGE

Fig. 4.

ID

ZDB-IMAGE-190723-2567

Genes

Antibodies

Ab-3G8

Publication

Skvarca et al., 2019 - Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish

All Figures

Figures for Skvarca et al., 2019

Image

Figure Caption

Fig. 4.

Neutrophil and macrophage populations change in the kidney field after AKI. (A-D) Tg(cdh17:mCherry); Tg(lyz:EGFP) transgenic zebrafish were used for neutrophil analyses. (E-H) Tg(cdh17:mCherry); Tg(mpeg1;dendra2) transgenic zebrafish were used for macrophage analyses. (A,E) Transgenic lines were injected with gentamicin at 3 dpf and imaged at 2 dpi. (B,F) Snapshots of live imaging of lyz+ neutrophils imaged at 1 dpi for 13.5 h (B) and mpeg1+ macrophages imaged at 2 dpi for 19.5 h (F) in a no-injury and gent-AKI setting. Arrows indicate neutrophils or macrophages adjacent to PTs. (C,G) Immunofluorescence co-stain of PTs (red or green) and neutrophils (green) (C) or macrophages (red) (G) in no-injury and gent-AKI at 3 dpi. PTs are outlined in white and adjacent leukocytes are marked with arrows. (D,H) Quantification of neutrophil (D) and macrophage (H) numbers adjacent to the PT before and after injury. Mean_NoInjury5dpf=4.24 (N=34) vs Mean_2dpi=7.76 (N=34) vs Mean_NoInjury6dpf=5.15 (N=34) vs Mean_3dpi=11.60 (N=48). Adjacent leukocytes were counted for both no injury and gent-AKI at 1, 2 and 3 dpi (D), and 2 and 3 dpi (H). Data pooled from three biological replicates are shown expressed as mean±s.e.m. One-way ANOVA: *P<0.05, **P<0.01, ***P<0.005. Scale bars: 20 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.