ZFIN Image: Kim et al., 2019, Figure 2

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Figure Caption

Figure 2

Effects of rice bran ash mineral extract (RBM) on pigmentation in zebrafish. (A) Synchronized zebrafish embryos (n = 20) were exposed to RBM, and pigmentation was observed under a stereomicroscope via inferior views of the embryos at 5 dpf; (B) Zebrafish melanin content was detected by melanin content assay at 5 dpf (n = 80); (C) Tyrosinase activity in zebrafish (n = 80) was detected by tyrosinase assay at 5 dpf; (D) Pigmentation density in the indicated white-outlined area was measured in treated embryos and normalized to that of control embryos using the software ImageJ. * p < 0.05, ** p < 0.01, compared to control. Scale bar; 500 µm.

Acknowledgments

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