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Figure 3
Confocal imaging of Tg(CMV:GFP-Lc3). Representative confocal images of GFP-Lc3 puncta (autophagosomes) in the trunk area of GFP-Lc3 transgenic zebrafish embryos injected with control morpholino or Hslbp3 translational-blocking morpholino and imaged at 2 days post fertilization (dpf) with or without pre-treatment with chloroquine (10 mM) for 6 h. Scale bars, 10 µM for the confocal images. Panel A, B shows the whole zebrafish larvae at 2 days post fertilization highlighting the trunk area chosen for confocal imaging; Panel A’, A’’, B’, B’’ shows respective confocal images.
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