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Figure 2
Strategy for establishing knock-in fish. Donor vector, gRNAs and Cas9 mRNA are injected into zebrafish embryos. The knock-in event is estimated by examining the expression of the fluorescent gene (green area). Potential F0 founders are mated with wild-type (WT) fish, and the knock-in lines expressing the fluorescent gene are selected. The targeted knock-in at the targeted locus is determined by genomic PCR and sequencing analysis.
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Full text @ Int. J. Mol. Sci.