ZFIN Image: Kawahara et al., 2016, Figure 1

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Figure 1

Targeted genomic modifications using genome editing technologies. DNA double-strand breaks (DSBs) induced by genome editing technologies are repaired by non-homologous end joining (NHEJ), microhomology-mediated end joining (MMEJ) and homologous recombination (HR). NHEJ repair, which connects the ends of the broken strands, leads to unpredictable insertion and/or deletion mutations (green bar), while MMEJ repair uses microhomology sequences (yellow box) and often causes a predictable small deletion. HR repair requires long double-strand DNA fragments (blue bar) that possess homology to the targeted genomic locus. Site-specific integrations of donor DNA are mediated by these DNA repair mechanisms.

Acknowledgments

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