ZFIN Image: Le et al., 2011, Figure 3

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Figure Caption

Figure 3 Direct binding of miR-125b to p53 network targets.

Biotinylated miR-125b was used as bait to pull-down mRNAs bound to miR-125b, using streptavidin-conjugated magnetic beads. The mRNAs were quantified by qRT-PCR, normalized to Gapdh, and then compared relative to the same mRNA species pulled down by a biotinylated C. elegans negative control microRNA. The enrichment of mRNAs bound to miR-125b is presented as mean log₂ fold change ± s.e.m. (n≥3 biological replicates). (A) Human: 13 out of 22 candidate targets were significantly enriched by miR-125b pull-down in human primary lung fibroblasts (hLF) 24 hours after transfection. (B) Mouse: 11 out of 13 candidate targets were significantly enriched by miR-125b pull-down in mouse 3T3 fibroblasts 24 hours after transfection. (C) Zebrafish: 8 of 14 candidate targets were significantly enriched by miR-125b pull-down in zebrafish embryos 24 hours after injection. Dashed line: cutoff for genes that were significantly enriched (Log₂ Fold change > 0.5, P<0.05).

Acknowledgments

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