ZFIN Image: Chen et al., 2019, Figure 1.

Image

Figure Caption

Figure 1.

Genome editing of gtpbp3 in zebrafish using CRISPR/Cas9 system. (A) Schematic representation of CRISPR/Cas9 target site at exon 2 of zebrafish gtpbp3 gene as used in this study. White boxes indicate coding region, black boxes indicate untranslated regions of exons and lines between exons indicate introns. The resultant truncated 98 aa non-functional protein caused by frame shifting deletion in gtpbp3 is shown in diagram. (B–D) Genotyping of gtpbp3^del14bp by Sanger sequence, the PAGE and Western blot analyses. (E) Lateral views of gtpbp3^+/+, gtpbp3^+/−, gtpbp3^−/− zebrafish at 5 dpf. (F) The ratios of genotypes/phenotype of offsprings (F2) in clutches from different F1 gtpbp3^+/- heterozygous crosses at 10 dpf (n = 42).

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.