ZFIN Image: Lin et al., 2016, Figure 3

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Figure Caption

Figure 3

Effect of human parathyroid hormone (PTH) amide on whole-body ECaC mRNA expression and calcium content in 3-dpf zebrafish embryos. One to two cell-stage zebrafish embryos were treated with 10 ng/mL human PTH 1-34 amide (H-5964, BACHEM, Torrance, CA, USA) for three days. The treatment of human PTH 1-34 amide resulted in significant increases in calcium content and ECaC mRNA expression. Whole-body Ca²⁺ content was measured by atomic absorption spectrophotometry. mRNA expression was analyzed by qPCR using the primer sets in the study by previous study [21], and the values were normalized to β-actin expression. Values are the mean ± SEM (n = 5). Student’s t-test, * p < 0.05.

Acknowledgments

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