ZFIN Image: Lee et al., 2015, Figure 5

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Figure Caption

Figure 5 In vivo validation of dsDMR enhancers

(a) Epigenome Browser^69,70 view of the six3a gene, neighboring dsDMR enhancers (six3a-e1, and six3a-e2, grey boxes) and cloned region for a negative control (six3a-ne1, and six3a-ne2, dark grey boxes). (b) GFP expression driven by the dsDMR enhancers of the six3a gene. (c) The methylation profiles of the six3a promoter and neighboring dsDMR enhancers (blue and red lines, left y-axis) and the expression profile of the six3a gene (green line, right y-axis). (d) Epigenome Browser view of the fgfr2 gene, neighboring dsDMR enhancers (fgfr2-e1, fgfr2-e2, fgfr2-e3, and fgfr2-e4, grey boxes) and cloned regions for negative controls (fgfr2-ne1, fgfr2-ne2, fgfr2-ne3, and fgfr2-ne4, dark grey boxes). (e) GFP expression driven by the dsDMR enhancers of the fgfr2 gene. (f) The methylation profiles of the fgfr2 promoter and neighboring dsDMR enhancers (blue and red lines, left y-axis) and the expression profile of the fgfr2 gene (green line, right y-axis). The asterisk * indicates that only one G1 transgenic line was established. Forebrain (f); midbrain hindbrain boundary (mh).

Acknowledgments

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