Fig. 6

Fig. 6

SRSF1 decoy oligonucleotides inhibit splicing and biological functions in glioblastoma cells. a Western blot of proteins pulled down with biotin conjugated SF2i2 (biSF2i2) or SCRM (biSCRM) using nuclear extracts from HEK293 cells overexpressing SRSF1. b Western blot of HEK293 cells co-transfected with pcDNA3 T7 SRSF1 and either SF2i2 or SCRM. Cells were fractionated and nuclear extract lysates were used for immunoprecipitation using anti-T7 antibody. Input and IPs were analysed by immunoblotting with the indicated antibodies. c RT-PCR and quantification of known splicing targets of SRSF1 in U87MG cells transfected with 2.5 µM of either SCRM, SF2i1, or SF2i2. Data represent means ± SD of six biological samples. d Western blot analysis of lysates from cells described in c. Data represent means ± SD of three biological samples. e RT-qPCR of p38 pathway target genes in cells described in c. Data represent means ± SD of triplicates. f RT-qPCR of NMD targets in cells described in c. *p-value ≤0.05, **p-value <0.004. Data represent means ± SD of triplicates. p-values were calculated using Student's t-test (two-tailed)