Fig. 1

Fig. 1

RBFOX1/2 decoy oligonucleotide binds RBFOX2 and affects its splicing targets. a Scheme representing inhibition of RNA binding proteins by decoy oligonucleotides (left panel). Motif sequences of decoy oligonucleotides (black letters) compared to consensus motif sequences of specific splicing factors (colored sequences) (right panel). b Western blot of proteins pulled down with biotin conjugated RBFOXi oligonucleotide (biRBFOXi) or SCRM (biSCRM) using nuclear extracts from HEK293 cells overexpressing RBFOX2. c Volcano plot showing statistically significant proteins pulled down with biotin conjugated RBFOXi, compared to SCRM control, using nuclear extracts from HEK293 overexpressing RBFOX2. d Western blot of HEK293 cells co-transfected with pcDNA3 Flag RBFOX2 and either RBFOXi or SCRM. Cells were fractionated and nuclear extract lysates were used for immunoprecipitation using anti-Flag antibody. Input and IPs were analysed by immunoblotting with the indicated antibodies. e RT-PCR and quantification of known RBFOX1/2 targets in U87MG cells transfected with 2.5 µM of either SCRM or RBFOXi. Gel image of representative experiment is shown above each graph. The same actin control is shown for all panels, as the gels are from the same experiment. **p-value <0.003, calculated using Student's t-test (two-tailed).  Data represent means ± SD of six independent biological samples