Fig. 5

Fig. 5

ZIPT-2.3 promotes zinc storage and IR-induced apoptosis. a, b ZIPT-2.3::GFP expression in wild type animals. Images are representative of three independent lines (a is at ×100 magnification and b is at ×630 magnification). c ZIPT-2.3::GFP in wild type and kri-1(ok1251) animals is measured along the intestinal midline from head to tail. Images are representative of three independent replicates (n ≥ 15). Scale bar is 50 μm. d Stored intestinal zinc measured in the intestines of wild type, kri-1(ok1251), kri-1(ok1251); mpk-2(ok219), kri-1(ok1251); klf-3(on34), zipt-2.3(ok2094), and pgp-2(kx48) animals (n ≥ 7). Scale bar is 25 μm. e Body cavity zinc measured in wild type, kri-1(ok1251), kri-1(ok1251); mpk-2(ok219), kri-1(ok1251); klf-3(on34), ttm-1(ok3505), and cdf-1(n2527) animals (n ≥ 25). Scale bar is 25 μm. f IR-induced germline apoptosis scored in wild type, kri-1(ok1251), kri-1(ok1251); mpk-2(ok219), kri-1(ok1251); let-60(ga89) and kri-1(ok1251); lip-1(zh15) animals after knock-down of zipt-2.3 (n ≥ 55). g IR-induced germline apoptosis scored in wild type, kri-1(ok1251), klf-3(on34), and kri-1(ok1251); klf-3(on34) animals after knock-down of zipt-2.3 (n ≥ 40). h IR-induced germline apoptosis scored in wild type, let-60(ga89), lip-1(zh15), and ced-9(n1653) animals grown on media supplemented with 1 mM zinc (n ≥ 35). d–h Red line is mean ± standard deviation. *P < 0.05, two-sided, unpaired t-test