ZFIN Image: Buchan et al., 2019, Fig 1

Image

Figure Caption

Fig 1

Transient expression of the lyz:MPO-mEmerald transgene labels zebrafish neutrophils.

A) Schematic of the lyz:MPO-mEmerald cmlc2:EGFP construct, which includes the neutrophil-specific promoter (lyz), the MPO gene with a C-terminal fluorescent tag (MPO-mEmerald) and a green heart marker to aid optimisation of transgenesis (cmlc2:EGFP). B) A zebrafish larva at 3 days post fertilisation (dpf), with the caudal haematopoietic tissue (CHT) indicated by the red box. C) The CHT of a double-transgenic Transient lyz:MPO-mEmerald,cmlc2:EGFP; Tg(lyz:nfsB-mCherry)sh260 larva with a population of neutrophils expressing both mEmerald and mCherry. The white arrowhead indicates the neutrophil enlarged below. D) Enlarged view of a neutrophil expressing mEmerald and mCherry.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One