Figure 4

Figure 4

Craniofacial assessment of zebrafish embryos. The zebrafish embryos were treated with Alcian blue and washed with acidic alcohol 3 dpf. The ratio of the width of the ceratohyal (x) to the tip of Meckel’s cartilage (y) was used as a measure of craniofacial defects. (a) Comparison of the x-to-y ratio between mutant and wild-type zebrafish embryos. The data are the mean ± SD. Statistical significance was derived using an unpaired t-test comparing the mutants with the corresponding WT. **p < 0.01; ****p < 0.0001; ns: not significant. NIC: No injection control. The number in bold represents the total count of zebrafish embryos in the experimental group. (b) Alcian-blue stained cartilage in the zebrafish. The x-axis shows the width of the ceratohyal, and the y-axis shows the length of the tip of Meckel’s cartilage. (c) Representative embryos with different x-to-y ratios.