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Figure 5—figure supplement 1.

ID: ZDB-IMAGE-190723-1077
Publication: Gálvez-Santisteban et al., 2019 - Hemodynamic-mediated endocardial signaling controls in vivo myocardial reprogramming
All Figures: Figures for Gálvez-Santisteban et al., 2019

Image

Figure Caption

Figure 5—figure supplement 1. <italic>klf2a</italic> and <italic>trpv4</italic> mutants display reduced endocardial Notch signaling.

(A–C) Confocal microscopy imaging performed on vmhc:mCherry-NTR, Tp1:d2GFP control hearts reveals reduced Tp1:d2GFP activation in (B) klf2a^-/- and (C) trpv4^-/- hearts at 24 hpt (6 dpf) when compared to (A) wild-type (wt) hearts (n = 15 control wt; 18 control trpv4^-/-; 20 control klf2a^-/-; 22 MTZ wt; 21 MTZ trpv4^-/-; 18 MTZ klf2a^-/-). (D–F) Whole-mount in situ hybridizations reveal reduced gene expression of notch1b in (E) klf2a^-/- (n = 4/19) and (F) trpv4^-/- (n = 14/29) vmhc:mCherry-NTR control hearts at 24 hpt (6 dpf) when compared to (D) wild-type (wt) hearts (n = 22/25). All confocal images shown are maximum intensity projections. V, ventricle; A, atrium; dpf, days post-fertilization; hpt, hours post-MTZ/DMSO treatment. Dashed lines outline the heart. Bars: 50 μm.

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