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Figure 4—figure supplement 1. Inhibiting hemodynamic flow perturbs post-injury re-activation of cardiac factors.

Whole-mount in situ hybridizations show increased (A) gata4 (n = 9/11), (E) hand2 (n = 10/10) and (I) nkx2.5 (n = 7/8) expression in vmhc:mCherry-NTR ventricle-ablated (ethanol/EtOH-treated) control hearts at 48 hpt (7 dpf). However, these cardiogenesis factors fail to increase after ventricle-ablation in (B, F, J) blebbistatin (Blebb) (n = 0/13 gata4; n = 1/10 hand2; n = 0/10 nkx2.5) or (C, G, K) tricaine (Tric) (n = 0/14 gata4; n = 2/10 hand2; n = 0/6 nkx2.5) treated hearts as well as in (D, H, L) gata2a-/- hearts (n = 5/17 gata4; n = 4/11 hand2; n = 2/7 nkx2.5). V, ventricle; A, atrium; dpf, days post-fertilization; hpt, hours post-DMSO treatment. Dashed lines outline the heart. Bars: 50 μm.

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