Figure 2—figure supplement 2.

Figure 2—figure supplement 2.

(A, B) Confocal imaging performed on (A) control and (B) ventricle-ablated klf2a:H2B-GFP; vmhc:mCherry-NTR; Tp1:nls-mCherry hearts at 24 hpt (6 dpf) shows co-expression of Tp1:nls-mCherry (red) in klf2a:H2B-GFP (green) activated cells (maximum intensity projections showed). (A’–A’’’’, B’–B’’’’) Single stacks of the projections in (A, B) show (A’’–B’’) co-expression of Tp1:nls-mCherry and klf2a:H2B-GFP (merged). (A’’-A’’’’-B’’, B’’’’) are magnifications of the area in the yellow dashed box in (A’, B’). (A’’, B’’) merge; (A’’’, B’’’) mCherry – Red or (A’’’’, B’’’’) GFP – Green. (C) Quantitation of the number of cells that display Tp1:nls-mCherry only (Tp1+/Klf2a-), Tp1:nls-mCherry and klf2a:H2B-GFP (Tp1+/Klf2a+) or total Tp1:nls-mCherry (Tp1+/Klf2a- and Tp1+/Klf2a+) in A, B) reveals that ventricle-ablated hearts have significantly more Tp1:nls-mCherry/klf2a:H2B-GFP-positive cells than control sham-ablated hearts at 24 hpt (6 dpf) (n = 10 each condition). V, ventricle; A, atrium; dpf, days post-fertilization; hpt, hours post-MTZ/DMSO treatment; dpt, days post-MTZ treatment. White dashed lines outline the heart. Bars: 50 μm. Mean + s.e.m. Student’s t test, **, p<0.01.