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GPRC5A depletion markedly increases caspase‐3 activation/PARP cleavage during hypoxia.
Three independent siRNA sequences targeting GPRC5A induce caspase‐3 activation/PARP cleavage during hypoxia.
GPRC5A depletion reduces hypoxic cell growth/survival. Crystal violet cell assays show reduced cell growth/survival in GPRC5A‐depleted cells during hypoxia (
Expression of an siRNA‐resistant GPRC5A cDNA rescues hypoxic GPRC5A‐depleted cells from apoptosis. Upper: doxycycline‐induced expression of GPRC5Asi1R rescues increased caspase‐3/PARP cleavage induced by GPRC5A depletion in hypoxia. Lower: generation of an siRNA1‐resistant GPRC5A cDNA by synonymous mutations.
GPRC5A depletion in hypoxia induces apoptosis as determined by the violet ratiometric membrane asymmetry probe/dead cell apoptosis assay and flow cytometry (
Caspase inhibitor QVD prevented caspase‐3 activation/PARP cleavage by GPRC5A depletion in hypoxia.