ZFIN Image: Alassaf et al., 2019, Figure 3—figure supplement 1

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Figure Caption

Figure 3—figure supplement 1—source data 1. Support cells are not affected by neomycin treatment.

(A) Mean percentage of surviving neuromast support cells at 4 hr post-neomycin treatment. To calculate support cell survival percentage, support cell number 4 hr post-neomycin treatment was normalized to mean support cell number in vehicle treated larvae in the same genotype. Two-way ANOVA, Holm-Sidak post test revealed no significant difference among groups. N = 6–9 larvae per group (shown at base of bars). Total number of neuromasts included in the analysis = 27 (wild type; vehicle-treated), 18 (wild type; 1 µM neomycin), 21 (wild type; 10 µM neomycin), 24 (wild type; 30 µM neomycin), 27 (pappaa^p170; vehicle-treated), 18 (pappaa^p170; 1 µM neomycin), 18 (pappaa^p170; 10 µM neomycin), and 18 (pappaa^p170; 30 µM neomycin). Error bars = SEM. (B) Representative confocal images of anti-SOX2 labeled support cells that were control or 30 µM neomycin treated. Scale = 10 µm.

10.7554/eLife.47061.008Figure 3—figure supplement 1—source data 1.Support cell survival post neomycin in wild type and <italic>pappaa<sup>p170</sup></italic>larvae.

Acknowledgments

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