ZFIN Image: Diamantopoulou et al., 2019, Figure 1—figure supplement 1

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Figure Caption

Figure 1—figure supplement 1—source data 1. Quantification of <italic>mbp</italic> expression around the posterior lateral line ganglion in <italic>adgrg6<sup>tb233c</sup></italic> mutants and wild-type sibling embryos.

(A) Top, wild-type (WT) sibling: bright-field image of 4 dpf embryo stained with mbp. Dorsal view, anterior to the left. Blue box indicates the region of interest (ROI) used for the quantification of the left and right posterior lateral line ganglion region. Below, three sets of images of the left and right ROI for each of 10 embryos (total of 20 areas), including the bright-field images, the threshold area (red) levels set with HSB on Fiji (Hue: 166–236, Saturation: 43–98, Brightness: 31–184), and the binary image used to determine the area of staining. (B) adgrg6^tb233c mutant embryo images comparable to those shown in A with identical threshold settings used in Fiji. (C) Comparison of the area of mbp staining between the adgrg6^tb233c mutant and wild-type sibling embryos using the quantification obtained in A and B. ****p<0.0001, Student’s t-test. Error bars represent the mean ±95% CI.

10.7554/eLife.44889.004Figure 1—figure supplement 1—source data 1.Source data for the percentage area of <italic>mbp</italic> expression shown in <xref rid='fig1s1' ref-type='fig'>Figure 1—figure supplement 1</xref>.

Acknowledgments

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