|
Fig. 6
LSN 2105786 treatment increases osteoblast differentiation. (A–F) Zns-5 antibody staining showed an expansion of the labeling area in the blastema region in 5 nM LSN 2105786 treated fins (E,F) compared to DMSO treated fins (B,C) at 2 dpa. Yellow boxed areas are shown in high magnification. (G–L) Zns-5 antibody staining of 7 dpa fins showed Zns-5 labeling in the lateral regions of the hemirays of DMSO treated fish (H,I), but 5 nM LSN 2105786 treated fish exhibited Zns-5 positive cells in the lateral regions of the hemirays and in the joints between the segments (K,L). Arrows point to the joints. (M–R) High magnification images of the boxed areas are shown in G–L. The 5 nM LSN 2105786 treated fins show Zns-5 labeling in the distal region of the bony rays (N,O) compared to DMSO treated fins (Q,R).