|
Fig. 1
Zebrafish Gsk3β is structurally and functionally equivalent to human GSK3Β. (A) Alignment of protein sequences of zebrafish and human gsk3β. Identical residue (*), conserved (:), and semi conserved (.) substitutions are marked. (B,C) 3D-protein structure of zebrafish (B) and human (C) gsk3β. (D–J) Live image of the 2 dpf control embryo (D). (E,F) Gsk3β translation blocking morpholino oligonucleotide (MO) injection (3.5 ng/µL) produced severe and moderate defects. Live images of the severely (E) and moderately (F) defective morphant embryos. (G,H) Live images of the embryos injected with zebrafish Gsk3β and human GSK3β mRNA. (I,J) Live images of the embryos co-injected with either MO or zebrafish Gsk3β mRNA or MO and human GSK3β mRNA. (K) Graph showing the percentage of phenotypes of the embryos in different conditions: MO alone, zebrafish Gsk3β mRNA alone, human GSK3β mRNA, MO + zebrafish Gsk3β mRNA, MO + human GSK3β mRNA.