ZFIN Image: Cagan et al., 2019, Fig. 1

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Figure Caption

Fig. 1

Examples of Tumors in Flies and Fish

(A) RAS^G12V; PTEN^−/− double mutant clones formed large glial-derived tumors in the fly brain. Red, repo (all glia); green, GFP (transformed glia). Insert shows the abnormally high density of glial-cell nuclei. From Read et al., 2009.

(B) High magnification view of a multi-target (involving four mutations) cancer model achieved by overexpressing RAS^G12V and using RNAi-mediated knockdown of PTEN, APC, and P53. Green, transformed hindgut cells; red, muscle cells; and blue, trachea. See Bangi et al., 2016.

(C) A transgenic zebrafish melanoma model. Expression of BRAF^V600E under the melanocyte-specific mitfa promoter resulted in stripe disruption and nevi. When crossed with p53-mutant fish, the resultant mitfa-BRAF^V600E;p53^−/− fish developed melanomas with 100% penetrance.

Acknowledgments

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Reprinted from Developmental Cell, 49, Cagan, R.L., Zon, L.I., White, R.M., Modeling Cancer with Flies and Fish, 317-324, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell