Fig. 3
The abundance of early PGCs promotes female development. a Representative images showing PGCs in wildtype and buc-overexpressed embryos at shield stage (PGCS was visualized by WISH). b Percentage of embryos with increased PGCs or normal number of PGCs in wildtype and buc-overexpressed embryos. c Representative images showing PGCs in wildtype and buc-overexpressed embryos at 1 dpf (PGCS was visualized using Tg(piwil1:egfp-UTRnanos3)ihb327Tg). d The sex ratio in population of wildtype and buc-overexpressed embryos. e RT-qPCR showing that buc was overexpressed in Tg(kop:KalTA4-UTRnanos3,CMV:EGFP)ihb8Tg/Tg(UAS:buc-UTRnanos3)ihb120Tg double-transgenic fish (dual-Tg) and Tg(UAS:buc-UTRnanos3)ihb120Tg. f The sex ratio in the populations of sibling control (Tg(kop:KalTA4-UTRnanos3,CMV:EGFP)ihb8Tg and wildtype fish), Tg(UAS:buc-UTRnanos3)ihb120Tg and double transgenics