ZFIN Image: Ando et al., 2019, Fig. 5

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Figure Caption

Fig. 5

Sox2 Expression in crbn-Overexpressing Embryos

(A) Embryos left uninjected or injected with mRNA encoding gfp, crbn^ΔMid, or crbn^WT were immunostained using anti-Sox2 antibody and counterstained with DAPI at 9 hpf. Bright-field (upper panels) and fluorescence (lower panels) images are shown. Animal pole to the top, dorsal side to the right. Dorsal epidermis (the early brain field) is indicated with asterisks.

(B) Illustration of 9-hpf embryos. Dorsal epidermis is indicated with dashed line. Lower panels in (C) correspond to the area indicated with red rectangle.

(C) Close-up views of the early brain field stained with anti-Sox2 antibody and counterstained with DAPI.

(D) Quantification and statistical analysis of the data shown in (C). DAPI-positive cells and Sox2-positive cells in the early brain field of 9-hpf embryos were counted and are shown as means ± SD (n = 15 per group).

Scale bar, 150 μm. ***p < 0.001.

Acknowledgments

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