ZFIN Image: Ando et al., 2019, Fig. 3

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Figure Caption

Fig. 3

Overexpression of crbn Enlarges the Head

(A–D) Head enlargement in crbn^WT-overexpressing embryos at the 10-somite stage (14 hpf) (A), 24 hpf (B), and 48 hpf (C and D). One-cell-stage embryos were left uninjected or injected with approximately 300 pg of capped mRNA (300 ng/μL) encoding gfp, crbn^ΔMid, or crbn^WT.

(E) One-cell-stage embryos were left uninjected or injected with capped mRNA at the indicated concentration. The head sizes of 14-hpf embryos were classified into “large” and “normal” based on the head-to-body ratio using the following criteria: ≤8%, normal; >8%, large. The fractions of large head embryos are shown as means ± SD (n = 3 per group).

(F and G) The ratios of eye diameter (F) and head thickness (G) to body length were determined at 48 hpf as in Figures 1B–1D and are shown as means ± SD (n = 15 per group).

Scale bar, 50 μm in (A and B). *p < 0.05, **p < 0.01, ***p < 0.001.

Acknowledgments

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