ZFIN Image: Breuer et al., 2019, Fig. S3

Image

Figure Caption

Fig. S3

Characterization of Qdprb1.

(A) Lateral views, anterior to the left of 72 hpf embryos. p53 knockdown does not rescue the microcephaly phenotype of Qdprb1 hypomorphic embryos. (B) RT-PCR confirms the predicted inclusion of intron 3 upon injection of the splice blocking MO resulting in a strong reduction of correctly spliced mRNA (RT-qPCR, C). (D). Qdprb1 morphant phenotypes using low concentrations of each MO and a combination of both showing a synergy effect between both. (E) Lateral view, 72hpf ATG MO Qdprb1 injected embryos reproduce the phenotype of Splice MO Qdprb1 hypomorphic embryos. (F) Lateral views, anterior to the left. Co-injection of qdprb1mRNA in Qdprb1 hypomorphic embryos rescues brain development.

Figure Data

Phenotype details

Acknowledgments

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