ZFIN Image: Evans et al., 2019, Fig. S2

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Figure Caption

Fig. S2

A J774 cells co-infected with a 50:50 mix of Δlac1-GFP and H99. Quantification of IPR for Δlac1-GFP cells within Δlac1-GFP:H99 2:1 or 1:2 co-infected macrophages. At least 20 co-infected macrophages were analysed for each condition over 4 experimental repeats. Student’s T test performed to compare ratios– 2:1 vs 1:2 * p = 0.012. B i Δlac1-GFP infected larvae imaged at 0, 1, 2 and 3 dpi. Fungal burden measured by counting GFP positive pixels in each larvae. At least 78 larvae measured per time point across 3 biological repeats. Box and whiskers show median, 5^th percentile and 95^th percentile. Unpaired Mann-Whitney U tests used to compare the burden between each strain for every time point, for p values see (S2A and S2B Fig). B ii Representative GFP images (representative = median value) of 2dpi Δlac1-GFP infected larvae, untreated at 0,1,2,3 dpi C i Δlac1-GFP Infected larvae treated with 10 μM prostaglandin E₂ or equivalent solvent (DMSO) control. At least 70 larvae measured per treatment group across 4 biological repeats. Box and whiskers show median, 5^th percentile and 95^th percentile. Unpaired Mann-Whitney U test used to compare between treatments, DMSO vs. 10 μM PGE₂ * p = 0.035. D i Δlac1-GFP Infected larvae treated with 10 μM 16,16-dimethyl prostaglandin E₂or equivalent solvent (DMSO) control. At least 75 larvae measured per treatment group across 4 biological repeats. Box and whiskers show median, 5^th percentile and 95^thpercentile. Unpaired Mann-Whitney U test used to compare between treatments, DMSO vs. 10 μM 16,16-dimethyl prostaglandin E₂ ns p = 0.062. E i Δlac1-GFP Infected larvae treated with 10 μM 15-keto-prostaglandin E₂ or equivalent solvent (DMSO) control. At least 58 larvae measured per treatment group across 3 biological repeats. Unpaired Mann-Whitney U test used to compare between treatments DMSO vs. 10 μM 15-keto-prostaglandin E₂ ns p = 0.50. C ii, D ii, E ii Representative GFP images (representative = median value) Δlac1-GFP infected larvae, at 2 dpi treated with 10 μM PGE₂ (C ii), 16,16-dm-PGE₂ (D ii) or 15-keto-PGE₂ (E ii).

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Acknowledgments

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