ZFIN Image: Evans et al., 2019, Fig. 3

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Figure Caption

Fig. 3

The observed activity of PGE₂ is due to its dehydrogenated derivative 15-keto-PGE₂: Fungal burden measured at 2 days post infection (2 dpi) by counting GFP positive pixels in each larvae.

A i H99-GFP Infected larvae treated with 10 μM 16,16-dimethyl-prostaglandin E₂ or equivalent solvent (DMSO) control. At least 75 larvae measured per treatment group from 4 biological repeats. Box and whiskers show median, 5^th percentile and 95^thpercentile. Unpaired Mann-Whitney U test used to compare between treatments, DMSO vs. 10 μM 16, 16-dm PGE₂ ns p = 0.9954. B i H99-GFP Infected larvae treated with 10 μM 15-keto-prostaglandin E₂ or equivalent solvent (DMSO) control. At least 55 larvae measured per treatment group from 3 biological repeats. Unpaired Mann-Whitney U test used to compare between treatments DMSO vs. 10 μM 15-keto = PGE₂ ** p = 0.0048 (threshold for significance 0.017, corrected for multiple comparisons). C i Δplb1-GFP Infected larvae treated with 10 μM 16, 16-dimethyl prostaglandin E₂ or equivalent solvent (DMSO) control. At least 45 larvae per treatment group from 3 biological repeats. Unpaired Mann-Whitney U test used to compare between treatments Δplb1-GFP DMSO vs 10 μM 16, 16-dm PGE₂ ns p = 0.98. D i Δplb1-GFP Infected larvae treated with 10 μM 15-keto-prostaglandin E₂ or equivalent solvent (DMSO) control. At least 35 larvae measured per treatment group from 2 biological repeats. Unpaired Mann-Whitney U test used to compare between treatments DMSO vs 10 μM 15-keto-PGE₂ * p = 0.0119 (threshold for significance 0.017, corrected for multiple comparisons). A ii, B iiRepresentative GFP images (representative = median value) H99-GFP infected larvae, at 2dpi treated with 10 μM 16,16-dm-PGE₂ (A ii) or 15-keto-PGE₂ (B ii). C ii, D iiRepresentative GFP images (representative = median value) Δplb1-GFP infected larvae, at 2dpi treated with 10 μM 16,16-dm-PGE₂ (C ii) or 15-keto-PGE₂ (D ii). E H99-GFP infected larvae treated with a combination of 3 μM AH6809 and 3 μM GW627368X or equivalent solvent (DMSO) control. Box and whiskers show median, 5^th percentile and 95^th percentile. At least 64 larvae measured per treatment group from 4 biological repeats. Mann-Whitney U test used to compare between treatments, no significance found.

Acknowledgments

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