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Fig. 3
Comparison of GFP‐labeled cephalic bones in Tg(ola.sp7:nlsGFP) zebrafish to alizarin red‐stained cephalic bones at 8 dpf. (A), Alizarin red stains mineralized bones in zebrafish head region, including cleithrum, opercle, branchiostegal rays, mandible, and notochord. (B), 3D‐reconstructed confocal image of Tg(ola.sp7:nlsGFP) zebrafish larvae. In comparison with alizarin red‐stained fish, eGFP is not expressed in the notochord of transgenic fish. CT, cleithrum; BR, branchiostegal rays; MD, mandible; NC, notochord
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