ZFIN Image: Denis et al., 2019, Fig. 5

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Figure Caption

Fig. 5

Cx40 contributes to EC proliferation in vivo. (A)Vascular development in Tg(flk1:eGFP) zebrafish embryos at 24 and 48 hpf. Intersegmental vessels (ISVs) sprout from the dorsal aorta (DA) and give rise to the dorsal longitudinal anastomotic vessel (DLAV). (B) Schematic representation of location of leo^t1 and leo^tq mutation in Cx41.8. (C,D) Cx41.8 and Cx45.6 expression in eGFP⁺ and eGFP^- cells isolated from Tg(flk1:eGFP) zebrafish embryos at 24 hpf was assessed by qPCR. N = 3. (E) Progress of ISV formation at 28 hpf in Tg(flk1:eGFP) (control; N = 7), Tg(flk1:eGFP)Cx41.8^tq/tq (N = 6), Tg(flk1:eGFP)Cx41.8^t1/t1 (N = 6) and Tg(flk1:eGFP)Cx41.8^t1/t1Cx45.6^-/- (N = 7) embryos. (F) Scores are: partial ISV sprouts (in black), complete ISVs (in gray) and ISVs with complete DLAV branching (in white). (G) Cx45.6 expression in eGFP⁺cells isolated from Tg(flk1:eGFP), Tg(flk1:eGFP)Cx41.8^tq/tq andTg(flk1:eGFP)Cx41.8^t1/t1 embryos at 24 hpf was assessed by qPCR. N = 3. (H)Examples of PH3 staining (in red) during ISV formation at 24 hpf in Tg(flk1:eGFP)and Tg(flk1:eGFP)Cx41.8^tq/tq embryos. Arrows indicate PH3⁺ cells. (I) PH3 positive nuclei in proximal ISVs of Tg(flk1:eGFP) (control N = 23) and Tg(flk1:eGFP)Cx41.8^tq/tq (N = 23) embryos at 24 hpf.

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Acknowledgments

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