Fig. 4

Il34 does not affect proliferation but does affect the distribution of YSMs to target organs. (A) NR⁺ microglia numbers in il34 mutants and their heterozygous and WT siblings at 5 dpf. (B) EdU/L-plastin staining of microglia in the optic tecti (dashed lines) of 4 dpf il34 mutants and WT controls, and quantification of microglia numbers, EdU⁺ microglia numbers and the fraction of EdU⁺ microglia among total numbers. (C) In vivo imaging of GFP⁺macrophages located on the yolk sac (dashed lines) in il34 mutants and WT controls, transgenic for mpeg1-GFP, and quantification at 48 hpf. YSMs with more than one protrusion were counted as branched YSMs. (D) In vivo imaging of mpeg1-GFP⁺ macrophages located in the head region (dashed lines) in il34 mutants and WT controls, and quantification at 48 hpf. (E) In vivo imaging of GFP⁺ macrophages located in the head region (dashed lines) in il34 mutants and WT controls, and quantification at 3 dpf. a, outline of the head region. (F) In vivo imaging of mpeg1-GFP⁺macrophages located in the tail (dashed lines) in il34 mutants and WT controls, and quantification. b, outline of the embryonic region/trunk region. Scale bars: 100 µm. *P<0.05, **P<0.01, ***P<0.001. One-way ANOVA and Student's t-test. Each dot represents one larva. Error bars represent s.d.