Fig. 5

Fig. 5

Analysis of maternal-zygote mutant and heterozygote cartilage ossification using specimens from the hdac4^aik3 line, stained using Alcian Blue and Alizarin Red dyes.

(A) Wild-type (non-sibling) larval pharyngeal skeleton at 7 dpf, lateral view. (B) Wild-type (non-sibling) larval pharyngeal skeleton at 12 dpf, lateral view. (C–E) maternal-zygotic mutant (C and D) and heterozygote (E) pharyngeal skeletons at 7 dpf showing premature ossification of the anguloarticular bone, ventral hypohyal, and ossification of the symplectic of the hyosymplectic (indicated by asterisk), lateral views. (F) Total scores of maternal-zygotic mutants and heterozygotes for premature/excessive ossification defects. (G and H) Wild-type posterior pharyngeal arches 3 and 4 at 7 dpf (G) and 12 dpf (H), including ossification of first and second ceratobranchial cartilages at 12 dpf, indicated by arrows, ventral views. (I) Maternal-zygotic mutant showing ossification of the first and second ceratobranchial cartilages cartilages at 7 dpf, indicated by arrows, ventral view. Abbreviations: aa, anguloarticular; cb1,2, ceratobranchial 1 and 2; q, quadrate; and hhv, ventral hypohyal. A–E, Scale bar = 200 microns, G–I, Scale bar = 50 microns. Cartilage is stained blue (Alcian Blue), bone is stained red (Alizarin Red). See Fig. 2A for schematic of the pharyngeal skeleton at 7 dpf.