ZFIN Image: Toselli et al., 2019, Fig. 5

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Figure Caption

Fig. 5

The number of islet endothelial cells decreases after beta-cell ablation. (a–g) Confocal projections of (a) 3 dpf, (b) 5 dpf control, (c) 5 dpf beta-cell ablated fish, (d) 8 dpf control, (e) 8 dpf beta-cell ablated fish, (f) 11 dpf control, (g) 11 dpf beta-cell ablated Tg(-1.2ins:htBid^TE-ON; -1.2ins:H2BmCherry; fli1:EGFP) fish; endothelial cells (green), beta-cells (red), glucagon (blue) and DAPI (grey). Scale bar = 10 μm. (h–j) The number of (h) beta-cells, (i) alpha-cells, and (j) endothelial cells during beta-cell ablation and regeneration in Tg(-1.2ins:htBid^TE-ON; -1.2ins:H2BmCherry; fli1:EGFP) fish treated with either DMSO (control) or DOX/TBF (beta-cell ablated) from 3–5 dpf. Box-and-whisker plots show median, and circles represent individual zebrafish. n = 5–12. Student’s t-test was conducted between the control and ablated groups at the same timepoint. #p < 0.0001, *p = 0.0004.

Acknowledgments

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